The effectiveness of Kersen Leaf extract (Muntingia calabura L.) on mice (Mus musculus L.) spermatozoa after D-Galactose induction
Purpose: This study aimed to test the effectiveness of kersen leaf extract (Muntingia calabura L.) on mouse spermatozoa (Mus musculus L.) after d-galactose induction.
Research Methodology: This study was conducted between September and October 2023 at the Zoology Laboratory of the University of Lampung. This study used a Completely Randomized Design (CRD) including five treatment groups. Each treatment included five mice: positive control (K0), negative control (K-) induced D-galactose 150 mg/kg body weight, treatment 1 (P1)-induced D-galactose 150 mg/kg body weight, Kersen leaf extract 35 mg/kg body weight, (P2) induced D-galactose 150 mg/kg body weight, and Kersen leaf extract 70 mg/kg body weight, and (P3) induced D-galactose 150 mg/kg body weight and Kersen leaf extract 105 mg/kg body weight for 35 days. Normal and homogeneous data were analyzed using analysis of variance (ANOVA) to determine whether the treatment's effect was significant. Differences were considered statistically significant at p < 0.05. Duncan's multiple interval post hoc test (=5%) was used to identify the most effective treatment.
Results: Analysis of Variance (ANOVA) showed that the total, viability, motility, and morphology of normal spermatozoa increased after induction with Kersen leaf extract at a dose of 105 mg/kg BB.
Limitations: This study provides readers with knowledge on natural antioxidants that prevent free radicals.
Contribution: This study provides readers with knowledge on natural antioxidants that prevent free radicals.